HA-Tag Tag Antibody is specific for the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors. HA-TAG Tag Antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting. In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.
Rabbit Polyclonal Antibody
Purified IgG - liquid
Keyhole limpet hemocyanin conjugated epitope tag peptide YPYDVPDYA (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
Phosphate buffered saline. 0.09% Sodium Azide (NaN3)
Store at +4ºC or at -20ºC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. For maximum recovery of the products, centrifuge the vial prior to opening the cap.
Applications & Suggested Dilutions
ELISA; Immunohistology - Paraffin (This product requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Citrate buffer is recommended for this purpose); Western Blotting