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EpiQuik 8-OHdG DNA Damage Quantification Direct Kit
A fast, accurate, and reliable solution for quantifying oxidative DNA damage (8-OHdG), trusted worldwide and supported by numerous citations.
High-Performance 8-OHdG Measurement Across Sample Types
This ELISA-style kit detects and quantifies oxidative DNA damage (8-OHdG) directly from DNA in under 4 hours.
- Fast and simple: Complete workflow in ~3 h 45 min.
- Convenient: Directly detects 8-OHdG from intact DNA, with no digestion or hydrolysis required.
- High sensitivity and specificity: Detects as little as 2 pg 8-OHdG with no cross-reactivity.
- Reliable and accurate: Includes optimized controls; results comparable to gold standard HPLC.
- Flexible and universal: Strip-well format; suitable for 8-OHdG quantitation from any species.
- Proven and trusted: Highly cited in over 60 peer-reviewed studies.
Whether you are analyzing a few samples or scaling up, the EpiQuik kit delivers consistent, dependable results with minimal hands-on time.
Compare 8-OHdG DNA Damage Quantification Kits
| Feature | EpiQuik 8-OHdG DNA Damage Quantification Direct Kit | Competitor A | Competitor B | Competitor C |
|---|---|---|---|---|
| Assay Principle | Direct 8-OHdG detection | Indirect antibody competition | Indirect antibody competition | Indirect antibody competition |
| Sensitivity | High (2 pg of 8-OHdG) | Low (590 pg) | Low (100 pg) | Low (30 pg) |
| Assay Control | Included positive and negative control oligos | Only 8-OHdG standard | Only 8-OHdG standard | Only 8-OHdG standard |
| Quantification | Reliable (relative + absolute) | Relative only | Relative only | Relative only |
| Specificity | High (no cross-reactivity; specific for 8-OHdG) | Very low (cross-reactivity with other DNA/RNA damage) | Very low (cross-reactivity with other DNA/RNA damage) | Very low (cross-reactivity with other DNA/RNA damage) |
| Fluorometric Version Available | Yes (cat #P-6004) | No | No | No |
Featured in the World's Leading Scientific Journals
The EpiQuik 8-OHdG DNA Damage Quantification Direct Kit is trusted by leading researchers and highly cited in top-tier publications with high impact factors.
Impact Factor: 17.7
Kang Y et al. (May 2022). Design of a two-dimensional interplanar heterojunction for catalytic cancer therapy. Nat Commun.
Impact Factor: 14.3
Li X et al. (Dec 2023). Non-canonical STING-PERK pathway-dependent epigenetic regulation of vascular endothelial dysfunction via integrating IRF3 and NF-kB in inflammatory response. Acta Pharm Sin B.
Impact Factor: 12.1
Chatterjee D et al. (Nov 2024). Maize unstable factor for orange1 encodes a nuclear protein that affects redox accumulation during kernel development. Plant Cell.
Impact Factor: 11.9
Toriumi et al. (Jun 2021). Combined glyoxalase 1 dysfunction and vitamin B6 deficiency in a schizophrenia model system causes mitochondrial dysfunction in the prefrontal cortex. Redox Biol.
Get Ready For Fast and Simple DNA Damage Quantification
View Product DetailsFrequently Asked Questions about the EpiQuik 8-OHdG DNA Damage Quantification Direct Kit
What is 8-OHdG and why is it a reliable marker of oxidative DNA damage?
8-hydroxy-2'-deoxyguanosine (8-OHdG, or 8-oxo-dG) is an oxidized form of deoxyguanosine generated by reactive oxygen species. Because it forms directly in DNA and is mutagenic, its abundance is widely used as a quantitative biomarker of oxidative DNA damage and oxidative stress.
How does the EpiQuik 8-OHdG Direct Kit work?
DNA binds to specially treated strip-wells and 8-OHdG is detected using capture and detection antibodies. The signal is enhanced and read colorimetrically at ~450 nm. Measured OD is proportional to the sample's 8-OHdG content.
What samples and how much input DNA do I need?
Use purified genomic DNA from any species (cells, tissues, FFPE, etc.). Recommended input is 100-300 ng DNA per reaction (about 300 ng optimal). DNA can be in water or TE buffer.
How long does the protocol take?
Approximately 3 h 45 min from start to finish.
What is the assay's limit of detection and specificity?
The kit detects as little as 2 pg 8-OHdG with high specificity and no cross-reactivity to 8-OHdG analogues.
Do I need to digest or hydrolyze DNA?
No. This is a direct assay that measures 8-OHdG in intact DNA, with no digestion, hydrolysis, or single-strand conversion needed.
What controls are included and how are results reported?
Universal positive and negative controls are included. Use the standard curve to calculate absolute 8-OHdG and report as pg 8-OHdG per ug DNA (or convert to 8-OHdG per 106 dG).
How do results compare with HPLC or LC-MS?
Measurements correlate strongly with HPLC/LC-MS while using a simpler microplate workflow.
Can I measure 8-OHdG directly in urine or serum?
This kit is designed for DNA inputs. For biofluids, use an assay intended for free 8-OHdG in fluids, or isolate DNA and run this kit.
How many samples fit on one plate and is it automation-friendly?
The 96-well strip-well format supports flexible layouts and high throughput; it is compatible with plate washers and liquid handlers.
What equipment do I need?
Standard ELISA tools: pipettes, plate washer (or manual washes), and a microplate reader at approximately 450 nm.
How should I store reagents and prepared samples?
Store components as indicated on labels. Keep DNA cold, avoid repeated freeze-thaw cycles, and protect light-sensitive reagents during handling.
