Methylamp Taq 5x PCR Mix Ready-to-Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
- Methylamp Taq DNA polymerase
- 5x Reaction Buffer: 1 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
- 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
- 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
- Blue dye: Migration equivalent to 3.5-4.5 kb DNA fragment
- Yellow dye: Migration rate in excess of primers in 1% agarose gel: <35-45 bp
- Compound that increases sample density for direct loading
- 1 ml of mix is sufficient for 250 reactions
- Suited for a wide range of PCR assays
- TA cloning
Routine storage: -20°C. Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8°C has no detrimental effects on the quality of Methylamp Taq 5x PCR Mix Ready-to-Load.