Methylamp H Minus M-MLV Reverse Transcriptase is a genetically modified M-MLV RT which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The enzyme has RNA polymerization-dependent and DNA polymerization-dependent activity but lacks ribonuclease H activity.
200 units/µl (one unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37°C).
- Methylamp H Minus M-MLV Reverse Transcriptase
- 5 x RT Reaction Buffer 1 (with MgCl2 and DTT): 0.25 M Tris-HCl, 0.5 M KCl, 30 mM MgCl2, 25 mM DTT
- 5 x RT Reaction Buffer 2 (Mg2+ and DTT free): 0.25 M Tris-HCl, 0.5 M KCl
- 25 mM MgCl2
- 20 mM MnCl2
- 100 mM DTT
- cDNA synthesis
- RNA analysis by primer extension
- DNA labeling
50% glycerol (v/v), 50 mM Tris-HCl pH 8.0 at 25ºC, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, 0.1% NP-40. Store at -20°C.