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Methylamp H Minus M-MLV Reverse Transcriptase

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Reagent Type: PCR Enzyme
100% Guarantee: 6 months
Catalog No.SizePriceQty
R12017-110000 Units $287.00  Discontinued
Availability: Discontinued 
Product Overview

Description
Methylamp H Minus M-MLV Reverse Transcriptase is a genetically modified M-MLV RT which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The enzyme has RNA polymerization-dependent and DNA polymerization-dependent activity but lacks ribonuclease H activity.

Concentration
200 units/µl (one unit is defined as the amount of enzyme required to catalyze the incorporation of  1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37°C).

Composition

  • Methylamp H Minus M-MLV Reverse Transcriptase
  • 5 x RT Reaction Buffer 1 (with MgCl2 and DTT): 0.25 M Tris-HCl, 0.5 M KCl, 30 mM MgCl2, 25 mM DTT
  • 5 x RT Reaction Buffer 2 (Mg2+ and DTT free): 0.25 M Tris-HCl, 0.5 M KCl
  • 25 mM MgCl2 
  • 20 mM MnCl2 
  • 100 mM DTT

Applications

  • cDNA synthesis 
  • RNA analysis by primer extension 
  • DNA labeling

Storage Conditions
50% glycerol (v/v), 50 mM Tris-HCl pH 8.0 at 25ºC, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, 0.1% NP-40. Store at -20°C.

User Guide & MSDS

[User Guide]*
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