DescriptionMethylamp Hot Taq EvaGreen® qPCR Mix (ROX) is optimized for real-time quantitative PCR assays. The ready-to-load mix includes Methylamp Hot Taq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. Only water, template and primers need to be added. Methylamp Hot Taq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
EvaGreen® Dye: DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!
ROX Dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR. Composition
Applications
Storage ConditionsRoutine storage: -20°C. Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Methylamp Hot Taq EvaGreen® qPCR Mix (ROX).