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Methylamp Hot Taq EvaGreen qPCR Capillary Mix

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Reagent Type: PCR Mix
100% Guarantee: 6 months
Catalog No.SizePriceQty
R12029-11 ml $143.00  Discontinued
Availability: Discontinued 
Product Overview

Description
Methylamp Hot Taq EvaGreen® qPCR Capillary Mix is optimized for real-time quantitative PCR assays. The ready-to-load mix includes Methylamp Hot Taq DNA polymerase, ultrapure dNTPs, MgCl2, and EvaGreen® dye. Only water, template and primers need to be added. Methylamp Hot Taq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Methylamp Hot Taq EvaGreen® qPCR Capillary Mix can be used with LightCycler® 1.x and LightCycler® 2.0 (Roche Applied Sciences).

Composition

  • Methylamp Hot Taq DNA polymerase
  • 5 x qPCR Buffer E
  • 12.5 mM MgCl2: 1 x PCR solution – 2.5 mM MgCl
  • dNTPs, including dTTP to improve reaction sensitivity and efficiency
  • EvaGreen® dye
  • BSA
  • 1 ml of mix is sufficient for 250 reactions

 Applications

  • Detection and quantification of DNA and cDNA targets including DNA from bisulfite treatment, ChIP or MeDIP
  • Profiling gene expression
  • Microbial detection
  • Viral load determination

Storage Conditions
Routine storage:-20°C. Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Methylamp Hot Taq EvaGreen® qPCR Capillary Mix.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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