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EpiSonic Multi-Functional Bioprocessor 1100

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For high throughput sonication of samples using PCR plates

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Complete setup of the EpiSonic 1100 sonicator (chiller is included as standard package)
Demonstration of different DNA shearing conditions. 2
Demonstration of consistent DNA shearing in different positions. 2
Demonstration of reproducible DNA shearing from run to run. 2
The Working Principle of the EpiSonic™ 1100 sonicator.
Purpose: Sample Fragmentation
Catalog No.SizePriceQty
EQC-11001 unit $0.00  Discontinued
 
Availability: Discontinued 
Product Overview

This unit has reached End-of-Life status and is no longer supported. We recommend upgrading to our EpiSonic 2000 Sonication System

The EpiSonic™ Multi-Functional Bioprocessor 1100 system, an upgrade from the previous EpiSonic 1000 model, is a multi-sample sonication instrument of the latest generation for use in a wide range of biological applications, in particular for DNA and chromatin shearing. This completely digital instrument allows for simultaneous optimized processing of 1 to 12 samples by tube, ideal for NGS applications, and can be easily integrated into existing lab workflows. Chiller is included at no additional charge.

Applications
The EpiSonic
 1100 sonicator was designed and built with the processing of biological samples specifically in mind, particularly NGS applications via Illumina or Ion Torrent workflows.

  • DNA Shearing
  • Chromatin Shearing
  • Cell Disruption/Lysis 

Features
The EpiSonic 1100 is a highly advanced ultrasonic instrument that incorporates Digitally Adaptive Sonocavitation™ (DAS) technology for efficiently processing biological samples with the following key features:

  • Touch Screen Control - Simple, modern operation with intuitive menu navigation
  • High Degree of Precision - Adjust all settings digitally; no dials or knobs
  • Programmable Memory - Store successful protocols for future use
  • Chilled Environment - Included cooling system recirculates chilled water to maintain sample integrity and prevent thermal degradation
  • Multi-Sample Processing - Simultaneously process up to 12 samples optimally with standard 0.2 ml PCR single tubes or up to 96 samples with 96-well PCR plates (Plate Rack sold separately)
  • Cost-Saving - Capable of handling common 0.2 ml PCR tubes or semi-skirted PCR plates, as well as low-priced EpiSonic-provided consumables
  • Shallow Waterbath - Less liquid medium allows for faster and highly focused sonication on samples
  • Closed Vessel Processing - Non-contact sonication in sealed tubes or wells prevents contamination and sample loss
  • Scalability - Simple, removable, and interchangeable vessel rack allows for expansion to different vessel sizes
  • Real-Time Energy Monitoring - Live screen output of amplitude, wattage, and joule readings
  • Real-Time Temperature Monitoring - Live screen output of temperature in sample reservoir (temperature probe required)
  • Improved Bio-Safety - No aerosol formation; no tedious manipulation of probes
  • Workflow Integration - Streamlines into multi-sample chromatin immunoprecipitation (ChIP) and massively parallel sequencing workflows
  • Automatic Overload Prevention - Detects faults and shuts down to prevent damage to circuits
  • No Moving Parts - Eliminates any possibility of mechanical failure or breakdown
  • Extensive Warranty - Backed by a 20 month warranty to ensure quality and customer satisfaction
High throughput sonication
Complete setup of the EpiSonic™ 1100 sonicator (chiller is included as standard package).

EpiSonic video demonstration
Video demonstration of the EpiSonic™ 1100 sonicator.

DNA shearing Bioanalyzer data
Fig. 1. 3 µg of placenta DNA in 0.2 ml tubes were sheared with the EpiSonic™ 1100 sonicator for different sonication durations (10, 15, 20, 45, and 62.5 min). The resulting lengths of the sheared DNA were analyzed with an Agilent Bioanalyzer 2100.
Product Details

Technological Advantages
Several new aspects have been introduced since the earlier 1000 model to enhance the performance of the EpiSonic.

  • The internal circuitry has been modified to apply DAS™ technology to allow for further precision and reproducibility
  • The redesigned, double-concave shaped sample processing horn causes piezoelectric vibration to propagate more evenly through the entire liquid medium (water) within the reservoir so that acoustic energy/mechanical stress is equally transmitted into samples located in different positions
  • A new vessel rack is conveniently included for pre-optimized operation with sample position consistency and run-to-run reproducibility
  • The included, new recirculating chiller promotes an isothermal environment to maintain the integrity of sensitive samples and prevent thermal degradation
  • Better optimized protocols included to shear DNA into various lengths for ideal compatibility with library preparation in next generation sequencing platforms from Illumina, Roche, and Life Technologies

Proven Precision, Consistency, & Reproducibility
The EpiSonic 1100 has been extensively tested and optimized for shearing samples in a precise, consistent, and reproducible manner. Desired sample sizes are able to be achieved due to the digital precision of the amplitude controls by allowing for user adjustment in intensity intervals of 1% at a time [Fig. 2]. Furthermore, samples placed in different positions with the included vessel holder (Tube Rack) are able to maintain consistency and equal sizes between each other [Fig. 3]. Most importantly, samples are sheared in reproducible lengths between the first run and the next run, creating predictable results and eliminating optimization time and labor [Fig. 4].

The Working Principle
The technology that drives the EpiSonic 1100 so well is Digitally Adaptive Sonocavitation™ (DAS), a principle that is ideal for shearing DNA or chromatin as well as for lysing cells and tissues. During the DAS process, the electronic signal produced by a digitally-controlled generator is converted into acoustic/mechanical energy through a piezoelectric converter (also known as a transducer). When the acoustic energy is produced in water, negative pressure is generated and causes the distance between the water molecules to exceed the maximum molecular distance necessary to hold liquid intact. Consequently, the liquid breaks down to create millions of cavitation bubbles.

Example

These cavitation bubbles expand in size through several acoustic cycles as more acoustic energy is introduced. The size of the cavitation bubbles and the corresponding cavitation energy can be manipulated and digitally controlled by adjusting fully scaled amplitude levels (1-100% in intervals of 1%) with a DAS™-based device such as the EpiSonic 1100. When the acoustic energy is suddenly removed, these cavitation bubbles collapse, creating intense shock waves in an extremely short period of time (microseconds), which transmit into vessels containing samples. This in turn forms highly targeted shearing forces to break up or disperse biomolecular samples including DNA, chromatin, and tissues in a non-contact, non-invasive manner. In a nutshell, a DAS™-based device such as the EpiSonic 1100 is able to shear samples with more precision and reproducibility than non-DAS™ instruments.

Compatible Sequencing Platforms
The EpiSonic 1100 can shear high quality DNA into any size under 2000 base pairs, which essentially covers all next-generation sequencing devices that require DNA library preparation. To be specific, some of the popular platforms that are compatible include the following:

  • Illumina Genome Analyzer
  • Illumina HiSeq
  • Illumina MiSeq
  • Roche 454 Sequencing GS FLX
  • Roche 454 Sequencing GS Junior
  • Life Technologies SOLiD System
  • Life Technologies Ion Torrent

Product Specifications

Model Number 1100 (also known as 1000E)
Input Voltage 100-120 VAC or 220-240 VAC @ 50/60 Hz
Power Rating 600 Watts
Program Memory 10 program slots
Operating Frequency 20 kHz
Timer Control Digital
Single Throughput Capability Yes
High Throughput Capability Yes
Chiller Dimensions  7.5"L x 5"W x 7"H (19 cm x 12.7 cm x 17.6 cm)
Generator Dimensions 19.5"L x 11"W x 5.25"H (49.5 cm x 27.9 cm x 13.3 cm)
Sound Enclosure Dimensions 10"L x 10"W x 16.5"H (25.5 cm x 25.5 cm x 42 cm)
Operating Environment Temperature: 39°-95°F (4°-35°C)
Relative Humidity 20-90% (Non Condensing)
For indoor use only
Origin of Manufacture USA
Warranty Period 20 months

Product Comparison

  EpiSonic™ Supplier D* Supplier C* Probe Sonication
Precise DNA Shearing Control Yes No No No
High Yields of Unbiased dsDNA Yes Yes N/A No
No. of Optimal Samples Per Run 12 12 1 0
No. of Max Possible Samples Per Run 177 12 1 1
Prevents Risk of Contamination Yes Yes Yes No
Digital Readouts and Touchscreen Display Yes No No No
Small Sample Volume
Permitted (<20 µl)
Yes No No No
Microplate Vessel Capability Yes No No Yes
Fits Into Existing Lab Workflows Yes Yes No Yes
Protocols Programmable
Into Internal Memory
Yes No No No
Reproducible Runs Yes N/A Yes No
Device Warranty 20 Months 12 Months 12 Months N/A
Cost Per Sample Run Low Medium (~5X more) High (~10X more) Very High (~40X more)

* Based on the most common models on the market.



Fig. 2. Demonstration of different DNA shearing conditions. 2 µg of placental DNA processed in 0.2 ml of PCR tube: (L) DNA ladder: 50-2000 bp; (1) Avg. 600 bp via 15 sec on 15 sec off for 20 cycles at 20% amplitude; (2) Avg. 400 bp via 15 sec on 15 sec off for 30 cycles at 20% amplitude; (3) Avg. 300 bp via 15 sec on, 15 sec off for 40 cycles at 20% amplitude; (4) Avg. 200 bp via 15 sec on 15 sec off for 90 cycles at 20% amplitude; (5) Avg. 150 bp via 20 sec on 30 sec off for 75 cycles; (U) Unsheared DNA.



Fig. 3. Demonstration of consistent DNA shearing in different positions. 2 µg of HeLa DNA in 0.2 ml PCR tubes were inserted into the 0.2 ml Tube Rack and processed with 20 sec on 30 sec off for 60 cycles at 20% amplitude. Average size of 250 bp is generated from DNA samples placed in the different positions: (L) DNA ladder; (1) Sample tube at Position 4C; (2) Position 4F; (3) Position 8G; (4) Position 8I; (5) Position 12C; (6) Position 12K; (U) Unsheared DNA.



Fig. 4. Demonstration of reproducible DNA shearing from run to run. 2 µg of placental DNA were separately sheared on four different days with the exact same sonication conditions (15 sec on 15 sec off for 30 cycles at 20% amplitude).
Product Components

Sonication Generator (100-120V) (Part #EQC-1100-PSG); or
Sonication Generator (220-240V) (Part #EQC-1100-PSG2) 
Sample Processing Horn (Part  #EQC-1100-PPH)
Soundproof Enclosure (Part  #EQC-1100-PSE)
Ultrasonic Converter (Part  #EQC-1100-PUC)
Recirculating Chiller (Part  #EQC-1100-PRC)
Power Cord (US) (Part  #EQC-1100-PPC); or
Power Cord (UK) (Part  #EQC-1100-PPCUK); or
Power Cord (Euro) (Part  #EQC-1100-PPCEU)
Flow Tubing with Fittings Set of 2 (Part  #EQC-1100-PFT)
Water Release Connector Set of 2 (Part #EQC-1100-PWR)
Converter Cable (Part  #EQC-1100-PCC)
Assembly Wrench Set of 2 (Part  #EQC-1100-PSW)
0.2 ml Tube Rack (Part  #EQC-1100-PTR)
Starter Vessel Pack 

Frequently Asked Q's

What is ultrasonics?
Ultrasonics, or ultrasound, literally means beyond sound -- sound above the human audible spectrum. Standard ultrasonic processors or sonicators operate at a nominal frequency of 20 kilohertz (kHz) or 20,000 cycles per second (cps). The automatic tuning feature of the EpiSonic 1100 actually moves the frequency within a small range during operation to optimize performance. The ultrasonic energy can then be converted by a pziezoelectric converter/transducer into acoustic energy to cause liquid cavitation through mechanical vibrations.

Why does the EpiSonic operate at 20kHz?
At lower range frequencies (20kHz to 100kHz), large cavitation bubbles are produced -- the collapse of which causes extreme hydraulic shearing forces and effects. Digitally Adaptive Sonocavitation™ (DAS), a 20kHz principle, allows for the size of the cavitation bubbles and the corresponding cavitation energy produced at low acoustic frequencies to be digitally controlled by dynamic amplitude adjustments of 1% to 100%. Therefore, DAS™ is more suitable for generating controllable shearing forces to break up cells and macromolecules such as DNA and chromatin in a non-contact, precise, and reproducible manner.  

What are the differences between the lower frequency Digitally Adaptive Sonocavitation (DAS) and higher frequency mechanisms for acoustic-based sonication?
DAS™ operates at 20kHz to cater to users who desire flexibility in being able to powerfully shear samples at fast speeds as well as to precisely shear samples at slower speeds. In frequencies of levels higher than 20kHz (e.g., medium frequency range of 100kHz to 1000kHz such as Adaptive Focused Acoustics™ or AFA), there is less time for the cavitation bubbles to grow. This unfortunately results in smaller bubbles and a corresponding reduction in cavitation energy with increased wait times to effectively shear samples. 100kHz to 1000 kHz frequencies are instead better suited for submicron particle removal and often used for breaking up the structure of organic compounds such as chlorophenol, TBT, and MTBE. Higher frequency sonication does not generally hold any advantages over standard 20kHz frequency sonication for shearing DNA or chromatin. The DAS™ technology of the EpiSonic 1100 provides maximum versatility by combining the powerful shearing forces of 20kHz frequency with digitally precise amplitude controls to finely adjust the intensity of such forces.

Can the EpiSonic 1100 be used with any other applications?
Although the EpiSonic 1100 was specifically designed and supported for DNA shearing, chromatin shearing, and cell disruption/lysis, it is capable of disaggregation, homogenization, cleaning, mixing, degassing, dispersion, cleavage, catalyzing reactions, hydrolyzation, extraction, dissolution, emulsification, comminution, synthesis, microencapsulation.

What kind of volume sizes can be used with the EpiSonic 1100 per sample?
The 0.2ml tube rack is included as standard for up to 60 µl of sample content per PCR tube (15 µl optimal). A 96-well plate rack is also offered as an optional accessory for plates at up to 60 µl of sample content per well (15 µl optimal). The unit is also capable of handling 1.5ml microtubes.

 

Adaptive Focused Acoustics is a trademark of Covaris, Inc.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

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Sanchez-Mut JV et. al. (May 2018). PM20D1 is a quantitative trait locus associated with Alzheimer's disease. Nat Med.

MacDonald B et. al. (March 2018). Molecular disruption through acid injection into waste activated sludge – A feasibility study to improve the economics of sludge dewatering Journal of Cleaner Production. 176:966-975.

Burress ED et. al. (October 2017). Phylogenomics of pike cichlids (Cichlidae: Crenicichla): the rapid evolution and trophic diversification of an incipient species flock. J Evol Biol.

Figliozzi RW et. al. (September 2017). Reversing thyroid hormone mediated repression of a HSV-1 promoter via computationally guided mutagenesis. J Cell Sci.

Farthing DE et. al. (June 2017). Comparing DNA enrichment of proliferating cells following administration of different stable isotopes of heavy water. Sci Rep. 7(1):4043.

Buxbaum NP et. al. (June 2017). In vivo kinetics and nonradioactive imaging of rapidly proliferating cells in graft-versus-host disease. JCI Insight. 2(12)

Mukherjee A et. al. (November 2016). Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks. J Vis Exp. (117)

Anderson OS et. al. (November 2016). Novel Epigenetic Biomarkers Mediating Bisphenol A Exposure and Metabolic Phenotypes in Female Mice. Endocrinology. :en20161441.

Weinhouse C et. al. (July 2016). Epigenome-wide DNA methylation analysis implicates neuronal and inflammatory signaling pathways in adult murine hepatic tumorigenesis following perinatal exposure to bisphenol A. Environ Mol Mutagen. 57(6):435-46.

Morris M et. al. (August 2015). Tau post-translational modifications in wild-type and human amyloid precursor protein transgenic mice. Nat Neurosci. 18(8):1183-9.

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