This unit has reached End-of-Life status and is no longer supported. We recommend upgrading to our EpiSonic 2000 Sonication System
The EpiSonic™ Multi-Functional Bioprocessor 1100 system, an upgrade from the previous EpiSonic™ 1000 model, is a multi-sample sonication instrument of the latest generation for use in a wide range of biological applications, in particular for DNA and chromatin shearing. This completely digital instrument allows for simultaneous optimized processing of 1 to 12 samples by tube, ideal for NGS applications, and can be easily integrated into existing lab workflows. Chiller is included at no additional charge.
ApplicationsThe EpiSonic™ 1100 sonicator was designed and built with the processing of biological samples specifically in mind, particularly NGS applications via Illumina or Ion Torrent workflows.
FeaturesThe EpiSonic™ 1100 is a highly advanced ultrasonic instrument that incorporates Digitally Adaptive Sonocavitation™ (DAS) technology for efficiently processing biological samples with the following key features:
Technological AdvantagesSeveral new aspects have been introduced since the earlier 1000 model to enhance the performance of the EpiSonic™.
Proven Precision, Consistency, & ReproducibilityThe EpiSonic™ 1100 has been extensively tested and optimized for shearing samples in a precise, consistent, and reproducible manner. Desired sample sizes are able to be achieved due to the digital precision of the amplitude controls by allowing for user adjustment in intensity intervals of 1% at a time [Fig. 2]. Furthermore, samples placed in different positions with the included vessel holder (Tube Rack) are able to maintain consistency and equal sizes between each other [Fig. 3]. Most importantly, samples are sheared in reproducible lengths between the first run and the next run, creating predictable results and eliminating optimization time and labor [Fig. 4].
The Working PrincipleThe technology that drives the EpiSonic™ 1100 so well is Digitally Adaptive Sonocavitation™ (DAS), a principle that is ideal for shearing DNA or chromatin as well as for lysing cells and tissues. During the DAS™ process, the electronic signal produced by a digitally-controlled generator is converted into acoustic/mechanical energy through a piezoelectric converter (also known as a transducer). When the acoustic energy is produced in water, negative pressure is generated and causes the distance between the water molecules to exceed the maximum molecular distance necessary to hold liquid intact. Consequently, the liquid breaks down to create millions of cavitation bubbles.
These cavitation bubbles expand in size through several acoustic cycles as more acoustic energy is introduced. The size of the cavitation bubbles and the corresponding cavitation energy can be manipulated and digitally controlled by adjusting fully scaled amplitude levels (1-100% in intervals of 1%) with a DAS™-based device such as the EpiSonic™ 1100. When the acoustic energy is suddenly removed, these cavitation bubbles collapse, creating intense shock waves in an extremely short period of time (microseconds), which transmit into vessels containing samples. This in turn forms highly targeted shearing forces to break up or disperse biomolecular samples including DNA, chromatin, and tissues in a non-contact, non-invasive manner. In a nutshell, a DAS™-based device such as the EpiSonic™ 1100 is able to shear samples with more precision and reproducibility than non-DAS™ instruments.
Compatible Sequencing PlatformsThe EpiSonic™ 1100 can shear high quality DNA into any size under 2000 base pairs, which essentially covers all next-generation sequencing devices that require DNA library preparation. To be specific, some of the popular platforms that are compatible include the following:
Product Specifications
Product Comparison
* Based on the most common models on the market.