BackgroundInvolved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
DescriptionMGMT Recombinant Monoclonal Antibody [10G7]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP16455
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human MGMT
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesMethylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase), MGMT
ApplicationELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200
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