BackgroundActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
DescriptionMCM3 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityHuman
IsotypeIgG
Uniprot IDP25205
PurificationAffinity Purified
ImmunogenRecombinant Human DNA replication licensing factor MCM3 protein (1-295AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesDNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3
ApplicationELISA, WB, IHC, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200