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HIST1H2AG Polyclonal Antibody

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All lanes: HIST1H2AG Polyclonal Antibody at 4ug/ml+HL-60 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 14kDa
Observed band size: 14kDa
Immunohistochemistry of paraffin-embedded human lung cancer using HIST1H2AG Polyclonal Antibody at dilution 1:100
Immunohistochemistry of paraffin-embedded human testis using HIST1H2AG Polyclonal Antibody at dilution 1:100
Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug HIST1H2AG Polyclonal Antibody or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the PGK1 promoter.
Immunofluorescent analysis of Hela cells using HIST1H2AG Polyclonal Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Application: ChIP, ELISA, IF, IHC, WB
Areas of Research: Unmodified Histone
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68306-02020 µg $59.00 
A68306-05050 µg $256.00 
A68306-100100 µg $359.00 
Availability: Usually Ships Within 1 Week 
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
HIST1H2AG Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P0C0S8

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Histone H2A type 1 protein (2-130AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H2A type 1, H2A.1, HIST1H2AG, H2AFP, HIST1H2AI, H2AFC, HIST1H2AK, H2AFD, HIST1H2AL, H2AFI, HIST1H2AM, H2AFN

Application
ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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