BackgroundSerine/threonine-protein kinase vital for cell cycle control, meiosis, and DNA duplication. Phosphorylates multiple targets including CTNNB1, p53/TP53, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Manages G1-S transition, DNA synthesis initiation, and coordinates mitosis/meiosis timing. Crucial for cellular balance in hESCs. Active during S and G2 phases, influenced by cyclin E and cyclin A2. Impacts gene silencing, DNA damage response, centrosome duplication, and more. Also engaged in nitric oxide signaling.
DescriptionPhospho-CDK2 (Y15) Recombinant Monoclonal Antibody [2C4]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP24941
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Phospho-CDK2 (Y15)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesCyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2
ApplicationELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000