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Histone H4K16ac (Acetyl H4K16) Monoclonal Antibody [RMC429A]

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Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue
All lanes: Histone H4K16ac (Acetyl H4K16) Monoclonal Antibody [RMC429A] at 1.65ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 KDa
Observed band size: 11 KDa
Immunocytochemistry analysis of Histone H4K16ac (Acetyl H4K16) Monoclonal Antibody [RMC429A] diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) with Histone H4K16ac (Acetyl H4K16) Monoclonal Antibody [RMC429A] at 1:403, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Hela cells stained with Histone H4K16ac (Acetyl H4K16) Monoclonal Antibody [RMC429A] (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Application: ELISA, FC, ICC, IF, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68404-05050 µl $249.00 
A68404-100100 µl $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
Histone H4K16ac (Acetyl H4K16) Monoclonal Antibody, clone RMC429A. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
P62805

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H4, HIST1H4A, H4/A, H4FA, AND, HIST1H4B, H4/I, H4FI, AND, HIST1H4C, H4/G, H4FG, AND, HIST1H4D, H4/B, H4FB, AND, HIST1H4E, H4/J, H4FJ, AND, HIST1H4F, H4/C, H4FC, AND, HIST1H4H, H4/H, H4FH, AND, HIST1H4I, H4/M, H4FM, AND, HIST1H4J, H4/E, H4FE, AND, HIST1H4K, H4/D, H4FD, AND, HIST1H4L, H4/K, H4FK, AND, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, AND, HIST2H4B, H4/O, H4FO, HIST4H4, Acetyl-Histone H4 (K16)

Application
ELISA, WB, ICC, IF, FC; Recommended dilution: WB: 1:500-1:2000, ICC: 1:50-1:500, IF: 1:50-1:200

VCU0006

User Guide & MSDS

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