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Histone H3R8 Dimethyl Asymmetric (H3R8me2a) Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R8 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R8 antibody.
Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R8 antibody. Blue: DAPI for nuclear staining.
Application: IF, IHC, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3716-05050 µl $253.00 
A-3716-100100 µl $399.00 
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Product Overview

Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.

Description
Histone H3R8 Dimethyl Asymmetric (H3R8me2a) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat, Broad Range

Isotype
IgG

Uniprot ID
Q16695

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human Asymmetric DiMethyl-Histone H3-R8

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
H3R8me2a, HIST1H3J, H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, HIST3H3, H3 Arginine 8 me2a

Application
WB, IHC, IF; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Customer Reviews

Rating by k**********@osu.edu Verified Purchase Reviewed on: Wednesday 29 March, 2017
Application Description
I used the antibody to determine the effect of PRMT5 knock down on global gene regulation in mantle cell lymphoma cells. We monitored symmetric dimethylation of histone H3R8 via Western blotting.

Other Thoughts
Good overall product.

Pros:
specificity was good. price was reasonable.

Cons:
What are the cons of the product? - nothing really.
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