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H3F3A (Ab-79) Polyclonal Antibody

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Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat spleen tissue
All lanes: H3F3A antibody at 0.73ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 KDa
Observed band size: 16 KDa
Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H3F3A or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Application: ChIP, ELISA, WB
Areas of Research: Histone Modification
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A70375-05050 µl $249.00 
A70375-100100 µl $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
H3F3A (Ab-79) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human, Rat

Isotype
IgG

Uniprot ID
P84243

Purification
Affinity purified

Immunogen
Peptide sequence around site of Di-methyl-Lys (79) derived from Human Histone H3.3

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H3.3, H3F3A, H3.3A, H3F3, PP781, AND, H3F3B, H3.3B

Application
ELISA, WB, ChIP; Recommended dilution: WB:1:200-1:2000

User Guide & MSDS

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