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CD4 Monoclonal Antibody [RMC935A]

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Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: CD4 Monoclonal Antibody [RMC935A] at 0.8ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
IHC image of CD4 Monoclonal Antibody [RMC935A] diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CD4 Monoclonal Antibody [RMC935A] diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application: ELISA, IHC, WB
Areas of Research: Immunology & Inflammation
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68428-05050 µl $249.00 
A68428-100100 µl $399.00 
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Product Overview

Background
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Description
CD4 Monoclonal Antibody , clone RMC935A. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
IgG

Uniprot ID
P01730

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4

Application
ELISA, WB, IHC; Recommended dilution: WB: 1:500-1:5000, IHC: 1:50-1:500

VCU0006

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