BackgroundInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
DescriptionCASP9 Recombinant Monoclonal Antibody [2D5]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP55211
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human CASP9
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesCaspase-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, Caspase-9 subunit p35, Caspase-9 subunit p10, CASP9, MCH6
ApplicationELISA, WB, IF, FC; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200