BackgroundPseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
DescriptionPhospho-MLKL (S358) Recombinant Monoclonal Antibody [4A6]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDQ8NB16
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Phospho-MLKL (S358)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesMixed lineage kinase domain-like protein, hMLKL, MLKL
ApplicationELISA, IHC; Recommended dilution: IHC:1:50-1:200