BackgroundInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Lacks activity is an dominant-negative inhibitor of caspase-9.
DescriptionCASP9 Recombinant Monoclonal Antibody [8G5]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP55211
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from Human CASP9
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesCaspase-9 (CASP-9) (EC 3.4.22.62) (Apoptotic protease Mch-6) (Apoptotic protease-activating factor 3) (APAF-3) (ICE-like apoptotic protease 6) (ICE-LAP6) [Cleaved into: Caspase-9 subunit p35, Caspase-9 subunit p10], CASP9, MCH6
ApplicationELISA, IF, FC; Recommended dilution: IF:1:50-1:200, FC:1:50-1:200