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BTAF1 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using BTAF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody. Western blot was performed from the immunoprecipitate using BTAF1 antibody at a dilition of 1:500.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using BTAF1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, IHC, IP, WB
Areas of Research: Transcription Factors
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-2725-05050 µl $189.00 
A-2725-100100 µl $299.00 
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Product Overview

Background
This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.

Description
BTAF1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
O14981

Purification
Affinity Purified

Immunogen
Recombinant Protein of Human BTAF1

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
MOT1, TAF172, TAFII170, TAF(II)170

Application
WB, IHC, IP, ChIP; Recommended dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50 ChIP 1:20 - 1:100

User Guide & MSDS

[User Guide]*
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