SOX10 Recombinant Monoclonal Antibody [8E1]

▲

▼

Immunofluorescence staining of Hela Cells with SOX10 rMAb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

IHC image of SOX10 rMAb diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of SOX10 rMAb diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
All lanes: SOX10 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 32 kDa
Observed band size: 60 kDa

Application:	ELISA, IF, IHC, WB
Areas of Research:	DNA Replication & Transcription
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A73783-050	50µl	$299.00
A73783-100	100µl	$399.00

Request A Bulk Quote

Earn up to 399 reward points for this purchase!

Order now & get it by Wednesday, December 10th

Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia (By similarity).

Description
SOX10 Recombinant Monoclonal Antibody [8E1]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P56693

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human SOX10

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Transcription factor SOX-10, SOX10

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

Product FAQ

No FAQs found at this time

Browse By Category

Products

Antibodies

Primary Antibodies

Cell Structure & Function Antibodies

Chromatin & Transcription Antibodies

Histone Modification Antibodies

Signal Transduction Antibodies

Metabolism Antibodies

Immunology & Inflammation Antibodies

Antibody Panel Packs

Instruments

Research Kits

DNA Methylation

Chromatin & Transcription

Histone Methylation

Acetylation & Deacetylation

Sample Preparation

Other Histone Modifications

Proteins & Enzymes

DNA Methylation/Demethylation Proteins

Modified Histone Proteins

Peptides

COVID-19 Assays

Services

DNA-based NGS Services

Chromatin-based NGS Services

qPCR-based Services

ELISA-based Services

Support

Technical Support

Quality Assurance

Resources

Corporate

Company Information

Procurement

Communication

SOX10 Recombinant Monoclonal Antibody [8E1]