DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
POLK Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
>95%, Protein G purified
Recombinant Human DNA polymerase kappa protein (227-337AA)
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
DNA polymerase kappa, DINB protein, DINP, POLK, DINB1
ELISA, WB; Recommended dilution: WB: 1:500-1:5000