EpiMag High Throughput DNA Isolation Universal Kit

The EpiMag™ High Throughput DNA Isolation Universal Kit utilizes magnetic bead technology for fast, cost-effective high throughput DNA isolation from various biological samples, including tissues, cultured cells, blood, bone marrow, mouse wash, buffy coat, urine, cerebrospinal fluid, buccal scrapes, sputum, bacteria, fungi, plants, and feces.  This kit has the following features and advantages:

• Optimized buffer chemistries for completely separating DNA from multiple materials, including various tissues, cultured cells, blood/bone marrows, buffy coat, urine, cerebrospinal fluid, buccal scrapes, feces, bacterial, fungi, and plants. 
• Cost-effective, fast, and straightforward procedure can be finished within 45 minutes for 96 samples and is highly amenable to various automation platforms. No gels or columns are needed.
• Efficient removal of protein, salts, enzymes, PCR inhibiting substances, and other impurities such as polysaccharides, polyphenols, and lipids.
• Sensitive and efficient DNA capture enables successful isolation with high recovery (>80% of input DNA), even when the quantities of starting material are limited.
• Manual and automation-friendly - scalable for single tube or 96-well plate formats.

Principle & Procedure
This kit contains all the optimized components needed for the simple and rapid isolation of PCR-ready genomic DNA from small quantities of various crude sample materials. The specifically designed magnetic beads allow for efficient and quick binding of the DNA to the beads after cell lysis. The DNA bound to the beads is pulled to the side wall of the well by applying a magnetic field (EpiMag HT magnetic separator (Q10002-1) or similar). DNA is purified by simply washing to remove any contaminants and potential PCR inhibitors. Purified DNA is then eluted from beads for use or storage.

Starting Materials
The amount of starting materials can be up to 1 X10^6 mammalian cells, 10 mg tissues, 30 μl or less of fresh anticoagulated blood (EDTA/ACD/citrate-treated) or bone marrow, 20 μl or less of buffy coat, 5 ml of fresh urine, 2 ml of fresh cerebrospinal fluid, buccal scrapes, 80 ul of saliva, 5 mg (wet weight) of bacterial or fungi, or 30–100 mg fresh plant leaf.