mTOR Polyclonal Antibody

Background
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

Description
mTOR Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH7.3.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
P42345

Purification
Affinity Purified

Immunogen
Synthetic peptide. Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR

Application
WB, IF/ICC, IP, ELISA; Recommended dilution: WB, 1:100 - 1:500; IF/ICC, 1:50 - 1:200; IP, 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.