BackgroundMTOR is a key serine/threonine protein kinase that centrally controls cellular functions like metabolism, growth, and survival in response to signals. It influences the phosphorylation of around 800 proteins directly or indirectly. It operates through two signaling complexes: mTORC1 and mTORC2. Activated mTORC1 enhances protein synthesis by phosphorylating factors in mRNA translation and ribosome production, including EIF4EBP1 which releases inhibition from translation initiation factor 4E. MTOR also activates RPS6KB1 and RPS6KB2, boosting protein synthesis via downstream targets like ribosomal protein S6, EIF4B, and the translation inhibitor PDCD4. It impacts pyrimidine biosynthesis and lipid synthesis. It regulates ribosome synthesis by phosphorylating and inhibiting MAF1, an RNA polymerase III repressor. MTORC1 interacts with autophagy, mitochondrial biogenesis, and growth factor signaling. In mTORC2, MTOR influences survival, cytoskeleton organization, and AKT1 activation at 'Ser-473', affecting cell growth. It also regulates osteoclastogenesis via CEBPB isoforms and contributes to various other cellular functions.
DescriptionPhospho-MTOR (S2481) Recombinant Monoclonal Antibody [3H11]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP42345
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Phospho-MTOR (S2481)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesSerine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
ApplicationELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200
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