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HUS1 Polyclonal Antibody

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Western blot
All lanes: HUS1 Polyclonal Antibody at 2ug/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 2: A431 whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 32, 30 kDa
Observed band size: 32 kDa
Immunohistochemistry of paraffin-embedded human gastric cancer using HUS1 Polyclonal Antibody at dilution of 1:100
Immunofluorescent analysis of A549 cells using HUS1 Polyclonal Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Application: ELISA, IF, IHC, WB
Areas of Research: DNA Damage & Repair
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A54483-02020 µg $99.00 
A54483-05050 µg $256.00 
A54483-100100 µg $359.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.

Description
HUS1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
O60921

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Checkpoint protein HUS1 protein (2-280AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
HUS1

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-2000, IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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