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Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody

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Citations (2) | Write a Review
Western blot analysis of extracts of various cell lines, using Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat AntiRabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Dot-blot analysis - Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody.
Immunofluorescence analysis of 293T cells using Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin embedded mouse testis using Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffinembedded rat testis using Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody at dilution of 1:200 (40x lens).
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, ChIPseq, IF, IHC, IP, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4048-02525 µl $147.00 
A-4048-05050 µl $285.00 
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Product Overview

Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

Description
Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Uniprot ID
P62805

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human TriMethyl-Histone H4-K20

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
H4K20me3 antibody; H4K20m3 antibody; HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3

Application
WB, IHC, IF, IP, ChIP, ChIPseq; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:50 - 1:200, ChIP 1:20 - 1:100, CHIPseq 1:20 - 1:100

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

Stixová L et. al. (January 2019). UVA irradiation strengthened an interaction between UBF1/2 proteins and H4K20 di-/tri-methylation. Chromosome Res.

Diehl F et. al. (March 2010). Cardiac deletion of Smyd2 is dispensable for mouse heart development. PLoS One. 5(3):e9748.

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