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Histone H2AK5ac (Acetyl H2AK5) Polyclonal Antibody

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Immunohistochemistry of paraffin-embedded human breast using H2AK5ac antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using H2AK5ac antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 degrees celsius for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37 degrees celsius for 18 hours. Blue: DAPI for nuclear staining.
Application: IF, IHC, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4300-05050 µl $253.00 
A-4300-10.1 ml $433.00  Discontinued
A-4300-100100 µl $399.00 
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Product Overview

Background
Histone proteins (H2A, H2B, H3, and H4) are the core components of a nucleosome and compact DNA into chromatin. Transcription regulation is affected depending on the availability of the chromatin structure, adjusted by post-translational modifications to histones – such as histone acetylation – and remodeling of nucleosomes. Histone acetyltransferases (HATs) act to acetylate histone H2A at lysine 5. 

Description
Histone H2AK5ac (Acetyl H2AK5) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.l.

Specificity
Mouse, Rat, Human

Isotype
IgG

Uniprot ID
P0C0S8

Purification
Affinity Purified

Immunogen
A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
HIST1H2AI; H2A/c; H2AFC; histone H2A type 1

Application
WB, IHC, IF; Recommended dilution: WB 1:500 - 1:2000; IHC 1:50 - 1:100; IF 1:50 - 1:100

User Guide & MSDS

[User Guide]*
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[Safety Data Sheet]
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