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HIST1H4A (Ab-20) Polyclonal Antibody

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IHC image of HIST1H4A (Ab-20) Polyclonal Antibody diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of HIST1H4A (Ab-20) Polyclonal Antibody diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with HIST1H4A (Ab-20) Polyclonal Antibody, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Application: ChIP, ELISA, IF, IHC
Areas of Research: Histone Modification
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A70376-05050 µl $249.00 
A70376-100100 µl $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
HIST1H4A (Ab-20) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300. 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P62805

Purification
Affinity purified

Immunogen
Peptide sequence around site of Lys (20) derived from Human Histone H4

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H4, HIST1H4A, H4/A, H4FA, AND, HIST1H4B, H4/I, H4FI, AND, HIST1H4C, H4/G, H4FG, AND, HIST1H4D, H4/B, H4FB, AND, HIST1H4E, H4/J, H4FJ, AND, HIST1H4F, H4/C, H4FC, AND, HIST1H4H, H4/H, H4FH, AND, HIST1H4I, H4/M, H4FM, AND, HIST1H4J, H4/E, H4FE, AND, HIST1H4K, H4/D, H4FD, AND, HIST1H4L, H4/K, H4FK, AND, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, AND, HIST2H4B, H4/O, H4FO, AND, HIST4H4

Application
ELISA, IHC, IF, ChIP; Recommended dilution: IHC:1:20-1:200, IF:1:20-1:200

User Guide & MSDS

[User Guide]*
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