Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
FZR1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Liquid. 0.03% Proclin 300. 50% Glycerol, 0.01M PBS, PH 7.4.
Protein G purified
Recombinant Human Fizzy-related protein homolog protein (1-493AA)
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Fzr, CDC20-like protein 1, hCDH1, Cdh1/Hct1 homolog, CDH1, FYR, FZR, KIAA1242, FZR1
ELISA, WB, IHC; Recommended dilution: WB:1:500-2000, IHC:1:20-1:200