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FICD Polyclonal Antibody

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Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, Rat kidney tissue
All lanes: FICD antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 KDa
Observed band size: 52 KDa
IHC image of FICD Polyclonal Antibody diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of FICD Polyclonal Antibody diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of PC3 cells with FICD Polyclonal Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC, WB
Areas of Research: Cell Metabolism
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human, Rat
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A70283-02020 µg $99.00 
A70283-05050 µg $249.00 
A70283-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified.

Description
FICD Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human, Rat

Isotype
IgG

Uniprot ID
Q9BVA6

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Adenosine monophosphate-protein transferase FICD protein (63-185AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Adenosine monophosphate-protein transferase FICD, AMPylator FICD, FIC domain-containing protein, Huntingtin yeast partner E, Huntingtin-interacting protein 13, HIP-13, Huntingtin-interacting protein E, FICD, HIP13, HYPE, UNQ3041/PRO9857

Application
ELISA, WB, IHC, IF; Recommended dilution: WB: 1:500-1:5000, IHC: 1:500-1:1000, IF: 1:50-1:200

User Guide & MSDS

[User Guide]*
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