DNMT1 Polyclonal Antibody

Background
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Description
DNMT1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
P26358

Purification
Affinity Purified

Immunogen
Recombinant protein (or fragment). Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; DNMT1

Application
WB, IHC-P, IF/ICC, IP, ELISA; Recommended dilution: WB, 1:500 - 1:1000; IHC-P, 1:50 - 1:200; IF/ICC, 1:50 - 1:200; IP, 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.