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DNA Methylation Antibody Panel Pack I

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Immunofluorescence staining with 5-methylcytosine antibody (anti-5mc) clone 33D3 (Cat#A-1014).
Dot blot analysis of Cat. #A-1018 with DNA oligos containing cytosine (C), 5-methylcytosine (5mC), or 5-hydroxymethylcytosine (5hmC). 10 ng of C, 5mC, and 5hmC DNA oligos each were spotted on a membrane. The membrane was incubated with 0.5 μg/ml of Epigentek's 5hmC Clone HMC/4D9 antibody (1:2000 dilution), then with peroxidase conjugated goat anti-mouse IgG (dilution 1:3000). Specific signal was only observed with 5-hmC DNA oligos. Fig. 2. Demonstration of high sensitivity and specificity of Epigentek's 5-hmC Clone HMC/4D9 antibody against 5-hmC DNA. Synthetic DNA oligos containing 5-hmC, 5-mC or only cytosine, respectively were added into the assay wells at different concentrations and then specific signals was only detected with 5-hmC DNA. Demonstration of high sensitivity and specificity of Epigentek's 5-hmC Clone HMC/4D9 antibody against 5-hmC DNA. Synthetic DNA oligos containing 5-hmC, 5-mC or only cytosine, respectively were added into the assay wells at different concentrations and then specific signals was only detected with 5-hmC DNA.
Application: DB, ELISA, ELISA, hMeDIP, IF, IHC, MeDIP, WB
Areas of Research: DNA Methylation
Clonality: Polyclonal
Conjugate: HRP, Unconjugated
Host: Goat, Mouse
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
C20000-12 x 25 µg $229.00 
Availability: Usually Ships in 3-4 Days 
Product Overview

Background
DNA methylation and demethylation are major epigenetic mechanisms that modulate gene expression in both eukaryotes and prokaryotes. Epigenetic modification 5-methylcytosine (5mC), considered the hallmark of gene silencing, has been implicated to play an important role in regulating several cellular and developmental processes, including mediating carcinogenic transformation of healthy cells. 5-hydroxymethylcytosine (5hmC) derived from enzymatic oxidization of 5mC has also been associated with many biological processes, including the pluripotency of embryonic stem cells, neuron development, and tumorigenesis in mammals.

Description
EpiGentek's Antibody Panel Packs provide a convenient way to investigate a panel of modifications using a collection of select antibodies. The DNA Methylation Antibody Panel Pack I is a complete set of 2 vials for the investigation of both 5-mC and 5-hmC, plus a bonus vial of secondary antibody is also included:

Product NameSizeApplicationsReactivity
5-Methylcytosine (5-mC) Monoclonal Antibody [33D3] 25 µg DB, ELISA, IF, IHC, IP, MeDIP Broad Range
5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9] 25 µg DB, ELISA, IF, IHC, hMeDIP Broad Range
HRP-Goat Anti-Mouse Secondary Antibody 50 µg ELISA, IF, IHC, WB Mouse

Storage
Varies. Please see user guide for recommended storage conditions for each antibody.

User Guide & MSDS

[User Guide]*
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