BackgroundMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
DescriptionATP5A1 Recombinant Monoclonal Antibody [5G11]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman, Mouse
IsotypeRabbit IgG
Uniprot IDP25705
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human ATP5A1
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesATP synthase subunit alpha, mitochondrial, ATP5A1, ATP5A ATP5AL2 ATPM
ApplicationELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200