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ANGPT1 Polyclonal Antibody

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All lanes: ANGPT1 Polyclonal Antibody at 2ug/ml+A549 whole cell lysate
Secondary
Goat polyclonal to Rabbit lgG at 1/10000 dilution
Predicted bands size: 58kDa
Observed bands size: 58kDa
Immunohistochemistry of paraffin-embeded human placenta at dilution of 1:50
Application: ELISA, IHC, WB
Areas of Research: Cell Growth, Signal Transduction
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A57770-02020 µg $99.00 
A57770-05050 µg $256.00 
A57770-100100 µg $359.00 
Availability: Usually Ships Within 1 Week 
Product Overview

Background
Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Description
ANGPT1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q15389

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Angiopoietin-1 protein (16-498AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
ANGPT1 KIAA0003

Application
ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

User Guide & MSDS

[User Guide]*
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