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EpiQuik Histone Demethylase (H3K4 Specific) Activity/Inhibition Assay Kit

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Schematic procedure for using the EpiQuik Histone Demethylase (H3K4 Specific) Activity/Inhibition Assay Kit.
HDM (H3-K4 specific) activity measured from Hela cell extracts.
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: Histone Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3074-4848 assays $338.00 
P-3074-9696 assays $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Histone Demethylase (H3-K4 Specific) Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure activity/inhibition of H3-K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase (H3-K4 Specific) activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria. The kit has the following advantages:

  • Fast procedure, which can be finished within 3 hours.
  • Innovative fluorescent assay with no use of radioactivity, extraction, and chromatography.
  • Direct measurement of HDM (H3-K4 specific) activity and inhibition without interference by thiol-containing chemicals such as DTT, GSH, and 2-mercaptoethanol.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Histone Demethylase (H3-K4 specific) Activity/Inhibition Assay Kit is designed for measuring total histone demetylase (H3-K4 Specific) activity/inhibition. In the assay with this kit, the unique di-methylated histone H3-K4 substrate is stably captured on the strip wells. Active HDMs bind to and demethylate histone H3-K4 substrate. The remaining un-demethylated substrate can be recognized with a high affinity anti-methylated histone H3-K4 antibody. The ratio or amount of the un-demethylated histone, which is inversely proportional to HDM enzyme activity, can then be fluorometrically quantified.

 
Fig. 1. Schematic procedure for using the EpiQuik Histone Demethylase (H3K4 Specific) Activity/Inhibition Assay Kit.

Fig. 2. HDM (H3-K4 specific) activity measured from Hela cell extracts.

Product Components

HD1 (10X Wash Buffer)
HD2 (HDM Assay Buffer)
HD3 (HDM Substrate)*
HD4 (HDM Standard, 20 µg/ml)*
HD5 (Capture Antibody, 100 µg/ml)*
HD6 (Detection Antibody, 200 µg/ml)*
HD7 (Fluoro-Developer)*
HD8 (Fluoro Enhancer)*
HD9 (Fluoro-Dilution)
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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