ALDOA Recombinant Monoclonal Antibody [7D8]

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Immunofluorescence staining of Hela Cells with ALDOA rMAb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0. 2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

IHC image of ALDOA rMAb diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0. 05% DAB.

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver whole cell lysate
All lanes: Aldolase antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 46 kDa
Observed band size: 40 kDa

Application:	ELISA, IF, IHC, WB
Areas of Research:	Glucose Metabolism
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell, Mouse
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human, Rat
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74414-050	50µl	$299.00
A74414-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).

Description
ALDOA Recombinant Monoclonal Antibody [7D8]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human, Mouse, Rat

Isotype
Rabbit IgG

Uniprot ID
P04075

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human Aldolase

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Fructose-bisphosphate aldolase A (EC 4.1.2.13) (Lung cancer antigen NY-LU-1) (Muscle-type aldolase), ALDOA, ALDA

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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ALDOA Recombinant Monoclonal Antibody [7D8]