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AIMP1 Polyclonal Antibody

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Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate
All lanes: AIMP1 antibody at 3.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35, 38 KDa
Observed band size: 35 KDa
IHC image of AIMP1 Polyclonal Antibody diluted at 1:200 and staining in paraffin-embedded human glioma cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of AIMP1 Polyclonal Antibody diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with AIMP1 Polyclonal Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC, WB
Areas of Research: Immunology & Inflammation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69867-02020 µg $99.00 
A69867-05050 µg $249.00 
A69867-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.

Description
AIMP1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q12904

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 protein (25-180AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, Endothelial monocyte-activating polypeptide 2, EMAP-2, Endothelial monocyte-activating polypeptide II, EMAP-II, Small inducible cytokine subfamily E member 1, AIMP1, EMAP2, SCYE1

Application
ELISA, WB, IHC, IF; Recommended dilution: WB: 1:500-1:5000, IHC: 1:200-1:500, IF: 1:50-1:200

User Guide & MSDS

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