BackgroundFunctions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
DescriptionAIFM1 Recombinant Monoclonal Antibody [4B2]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman, Rat
IsotypeRabbit IgG
Uniprot IDO95831
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human AIF
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesApoptosis-inducing factor 1, mitochondrial (EC 1.1.1.-) (Programmed cell death protein 8), AIFM1, AIF PDCD8
ApplicationELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200