BackgroundCell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed:9575153). Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed:22385435). Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT)
DescriptionTK1 Recombinant Monoclonal Antibody [6F6]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP04183
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from Human TK1
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesThymidine kinase, cytosolic (EC 2.7.1.21), TK1
ApplicationELISA, WB, IF, FC; Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200, FC:1:50-1:200