SLC2A3 Recombinant Monoclonal Antibody [14B1]

▲

▼

Overlay Peak curve showing MCF7 cells stained with SLC2A3 rMAb (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0. 2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of SLC2A3 rMAb diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0. 05% DAB.

IHC image of SLC2A3 rMAb diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0. 05% DAB.

IHC image of SLC2A3 rMAb diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0. 05% DAB.

Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: SLC2A3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 55-70 kDa

Application:	ELISA, FC, IHC, WB
Areas of Research:	Glucose Metabolism
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74599-050	50µl	$299.00
A74599-100	100µl	$399.00

Request A Bulk Quote

Earn up to 399 reward points for this purchase!

Order now & get it by Tuesday, December 16th

Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport.

Description
SLC2A3 Recombinant Monoclonal Antibody [14B1]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P11169

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human SLC2A3

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Solute carrier family 2, facilitated glucose transporter member 3 (Glucose transporter type 3, brain) (GLUT-3), SLC2A3, GLUT3

Application
ELISA, WB, IHC, FC; Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, FC:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

Product FAQ

No FAQs found at this time

Browse By Category

Products

Antibodies

Primary Antibodies

Cell Structure & Function Antibodies

Chromatin & Transcription Antibodies

Histone Modification Antibodies

Signal Transduction Antibodies

Metabolism Antibodies

Immunology & Inflammation Antibodies

Antibody Panel Packs

Instruments

Research Kits

DNA Methylation

Chromatin & Transcription

Histone Methylation

Acetylation & Deacetylation

Sample Preparation

Other Histone Modifications

Proteins & Enzymes

DNA Methylation/Demethylation Proteins

Modified Histone Proteins

Peptides

COVID-19 Assays

Services

DNA-based NGS Services

Chromatin-based NGS Services

qPCR-based Services

ELISA-based Services

Support

Technical Support

Quality Assurance

Resources

Corporate

Company Information

Procurement

Communication

SLC2A3 Recombinant Monoclonal Antibody [14B1]