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PHF10 Polyclonal Antibody

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Western blot
All lanes: PHF10 Polyclonal Antibody at 8ug/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: A2780 whole cell lysate
Lane 4: U251 whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: A375 whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 57, 56, 52 kDa
Observed band size: 56 kDa
Immunohistochemistry of paraffin-embedded human testis using PHF10 Polyclonal Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using PHF10 Polyclonal Antibody at dilution of 1:100
Immunofluorescent analysis of Hela cells using PHF10 Polyclonal Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Application: ELISA, IF, IHC, WB
Areas of Research: Chromatin Remodeling
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A60254-02020 µg $59.00 
A60254-05050 µg $256.00 
A60254-100100 µg $359.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Description
PHF10 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q8WUB8

Purification
>95%, Protein G purified

Immunogen
Recombinant Human PHD finger protein 10 protein (151-360AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
PHD finger protein 10, PHF10, BAF45A, BRG1-associated factor 45a, BAF45a, XAP135

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-2000, IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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