MMP9 Recombinant Monoclonal Antibody [29C11]

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Overlay histogram showing Jurkat cells stained with MMP9 rMAb (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0. 3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of MMP9 rMAb diluted at 1:235 and staining in paraffin-embedded human breast cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Application:	ELISA, FC, IHC
Areas of Research:	Other Primary Antibodies
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74237-050	50µl	$299.00
A74237-100	100µl	$399.00

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Product Overview
Datasheet & SDS
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Product Overview

Background
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Description
MMP9 Recombinant Monoclonal Antibody [29C11]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P14780

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human MMP9

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP9, CLG4B

Application
ELISA, IHC, FC; Recommended dilution: IHC:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
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MMP9 Recombinant Monoclonal Antibody [29C11]