BackgroundThis serine/threonine kinase is a vital component of the MAP kinase signaling pathway, which includes MAPK1/ERK2 and MAPK3/ERK1. The cascade has a multifaceted role in cell processes like growth, survival, differentiation, and more. It operates by regulating transcription, translation, and cytoskeletal organization. This pathway influences meiosis, mitosis, and postmitotic functions, impacting over 160 substrates including transcription factors, cytoskeletal elements, apoptotic regulators, and translation controllers. It also engages in endosomal dynamics and Golgi fragmentation. Protein kinases and phosphatases are among its substrates, broadening its signal specificity. It phosphorylates TPR upon EGF stimulation, participates in spindle assembly checkpoint, and influences PML degradation. It also phosphorylates CDK2AP2.
DescriptionMAPK1 Recombinant Monoclonal Antibody [10C7]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman, Mouse, Rat
IsotypeRabbit IgG
Uniprot IDP28482
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human ERK2
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesMitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2), MAPK1, ERK2 PRKM1 PRKM2
ApplicationELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200