HSP90AA1/HSP90AB1 Recombinant Monoclonal Antibody [10D6]

Background
This molecule serves as a chaperone, aiding in the maturation, maintenance, and precise regulation of specific target proteins vital for tasks like cell cycle control and signal transduction. Through its ATPase activity-driven cycle, it induces conformational changes in client proteins, activating them. It collaborates with co-chaperones, influencing substrate recognition, ATPase cycle, and chaperone function. Co-chaperones also connect it to various client protein types, acting as adapters. The cycle starts with ATP and co-chaperone recruitment, followed by client protein binding, forming a functional chaperone. Once the client protein is properly folded, it and the co-chaperone leave the HSP90 chaperone in a partially open, ADP-bound state, prepared for the next cycle. Beyond chaperone activity, it affects transcription machinery regulation at multiple levels. It impacts transcription factor levels, modifies epigenetic activity, and participates in histone eviction, activating gene expression. It additionally binds bacterial lipopolysaccharide (LPS), promoting LPS-induced inflammation and TNF secretion. Furthermore, it counteracts STUB1-mediated inhibition of TGF-beta signaling by inhibiting SMAD3 ubiquitination and degradation.

Description
HSP90AA1/HSP90AB1 Recombinant Monoclonal Antibody [10D6]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human, Rat

Isotype
Rabbit IgG

Uniprot ID
P07900/P08238

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human HSP90AA1/HSP90AB1

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38, HSP90AA1, HSP90A, HSPC1, HSPCA, Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, HSP 84, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Application
ELISA, WB, FC, IP; Recommended dilution: WB:1:500-1:5000, IP:1:200-1:1000