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HMGB1 Polyclonal Antibody

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Western blot
All lanes: HMGB1 Polyclonal Antibody at 10 ug/ml
Lane 1: Raji whole cell lysate
Lane 2: MCF7 whole cell lysate
Lane 3: HL60 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 24, 40 kDa
Western Blot
Positive WB detected in: mouse brain
All lanes: HMGB1 Polyclonal Antibody at 3.3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Immunohistochemistry of paraffin-embedded human tonsil tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human prostate cancer at dilution 1:100
Application: ELISA, IHC, WB
Areas of Research: Chromatin Remodeling
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A51497-02020 µg $59.00 
A51497-05050 µg $256.00 
A51497-100100 µg $359.00 
Availability: Usually Ships Within 1 Week 
Product Overview

Background
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Description
HMGB1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
P09429

Purification
>95%, Protein G purified

Immunogen
Recombinant Human High mobility group protein B1 protein (8-179AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Application
ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

User Guide & MSDS

[User Guide]*
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