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Histone H2A.Z Polyclonal Antibody

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Immunohistochemistry of paraffin-embedded mouse kidney using Histone H2A.Z Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Histone H2A.Z Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using Histone H2A.Z Polyclonal Antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using H2AFZ antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 30s.
Application: IHC, WB
Areas of Research: Histone Acetylation Antibodies
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A72908-05050 µl $256.00 
A72908-100100 µl $359.00 
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Product Overview

Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.

Description
Histone H2A.Z Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
P0C0S5

Purification
Affinity Purification

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human H2AFZ (NP_002097.1).

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Alternative Names
H2AFZ; H2A.Z-1; H2A.z; H2A/z; H2AZ; histone H2A.Z

Application
WB, IHC; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:200

User Guide & MSDS

[User Guide]*
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