BackgroundHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
DescriptionHistone H2AX Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityHuman, Mouse, Rat, Other (Wide Range)
IsotypeIgG
Uniprot IDP16104
PurificationAffinity Purified
ImmunogenA synthetic phosphorylated peptide around S2 of human Histone H2AX (NP_002096.1)
StorageShipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles
Alternative NamesH2AFX; H2A.X; H2A/X; H2AX; histone H2AX
ApplicationWB, IHC, IF; Recommended dilution: WB 1:500 - 1:1000, IHC 1:50 - 1:100, IF 1:50 - 1:100