Phospho Histone H2AX (S139) Monoclonal Antibody [RMC097A]

Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Description
Phospho Histone H2AX (S139) Monoclonal Antibody [RMC097A]. Unconjugated. Raised in: Rabbit.

Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. .

Specificity
Human

Isotype
IgG

Uniprot ID
P16104

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX; H2AXS139; H2AXS139ph

Application
ELISA, WB; Recommended dilution: WB:1:3000-1:10000