FOXO3 Recombinant Monoclonal Antibody [1E2]

Background
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription.

Description
FOXO3 Recombinant Monoclonal Antibody [1E2]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
O43524

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human FOXO3

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Forkhead box protein O3, AF6q21 protein, FOXO3

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200