EpiQuik HDAC2 Assay Kit (Colorimetric)

The EpiQuik™ HDAC2 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of HDAC2 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful HDAC2 assay experiment without the need for electrophoresis and transfer process. It is suitable for measuring HDAC2 level from various fresh tissues and cultured mammalian cells. The kit has the following advantages:

• Fast procedure, which can be finished within 3 hours.
• Innovative colorimetric assay to semi-quantitatively measure HDAC2 amount without the need for electrophoresis.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ HDAC2 Assay Kit is designed for measuring total HDAC2 amount from tissues or cells. In an assay with this kit, the nuclear proteins containing HDAC2 are stably coated on the strip wells. The HDAC2 is recognized with a high-affinity specific antibody. The amount of HDAC2 can be quantified through a HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

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